Assay

Neutrophil Oxidative burst

Key Words

Neutrophil oxidative burst

Specimen Collection

Whole blood (red EDTA)

Please contact the Lab (x8454) prior to collecting the bloods

Turnaround time

5 Days

This test is referred to another centre:

Department of Clinical Chemistry and Immunology

Level 4

Addenbrooke’s Hospital NHS Trust

Hills Road

Cambridge

CB2 2QQ

01223 217215

Test indications

This test is used in the investigation of patients suspected to have Chronic Granulomatous disease.

Presentation usually occurs in childhood with:

1.     Deep seated infection (Liver, perirectal or lung abscess, adenitis; or osteomyelitis) typically due to catalase-negative organisms such as Staphylococcus aureus, Serratia marcescens, or Aspergillus.

2.     Granulomatous disease in the gastrointestinal tract may mimic inflammatory bowel disease.

3.     Granulomata in respiratory, gastrointestinal or urogenital tracts may cause obstruction.

Methodology

Flow cytometry

Interferences

Sample storage and transport conditions can significantly affect cell viability, in particular samples must not be refrigerated. Concurrent sepsis or hyperglycaemia will interfere with this assay.

Reference Range

N/A

Analytical error

Contact referral laboratory

Reference change value

 N/A