T & B Subsets
|
Assay |
T & B Subsets |
|
Key Words |
T and B subsets, Lymphocyte, T cell, B cell, immunodeficiency |
|
Specimen Collection |
3.4ml EDTA (Red lid). 1.3ml EDTA for paediatric (Red lid). Do not refrigerate & must be less than 24 hours old on receipt (this is due to white cell degradation).
Samples arriving on Friday or on a weekday that preceeds a Bank Holiday must arrive in the laboratory before 12pm or it will NOT be processed |
|
Turnaround time |
3 working days |
|
Test indications |
Suspected immunodeficiency, in conjunction with immunoglobulin profile. |
|
Interferences |
Haemolysis, clotted samples |
|
Reference Range |
See tables below. Adult reference ranges from: Jentsch-Ullrich, K., et al. (2005). Lymphocyte subsets' reference ranges in an age- and gender-balanced population of 100 healthy adults—A monocentric German study. Clinical Immunology, 116(2), pp.192-197. Paediatric reference ranges from: Shearer, W., et al. (2003). Lymphocyte subsets in healthy children from birth through 18 years of age. Journal of Allergy and Clinical Immunology, 112(5), pp.973-980. |
|
Minimum retesting interval |
T & B Subset requests made within 14 days of a previous request on a patient are intervened. |
|
Additional information |
This test is currently UKAS accredited. |
|
Age |
Cells per microlitre (% of cells) |
||||
|
CD3+ |
CD3+ CD4+ |
CD3+ CD8+ |
CD19+ |
CD3- CD16+ 56+ |
|
|
0 – 3 months |
2500-5500 |
1600-4000 |
560-1700 |
300-2000 |
170-1100 |
|
(53-84) |
(35-64) |
(12-28) |
(6-32) |
(4-18) |
|
|
3 – 6 months |
2500-5600 |
1800-4000 |
590-1600 |
430-3000 |
170-830 |
|
(51-77) |
(35-56) |
(12-23) |
(11-41) |
(3-14) |
|
|
6 – 12 months |
1900-5900 |
1400-4300 |
500-1700 |
610-2600 |
160-950 |
|
(49-76) |
(31-56) |
(12-24) |
(14-37) |
(3-15) |
|
|
1 – 2 years |
2100-6200 |
1300-3400 |
620-2000 |
720-2600 |
180-920 |
|
(53-75) |
(32-51) |
(14-30) |
(16-35) |
(3-15) |
|
|
2 – 6 years |
1400-3700 |
700-2200 |
490-1300 |
390-1400 |
130-720 |
|
(56-75) |
(28-47) |
(16-30) |
(14-33) |
(4-17) |
|
|
6 – 12 years |
1200-2600 |
650-1500 |
370-1100 |
270-860 |
100-480 |
|
(60-76) |
(31-47) |
(18-35) |
(13-27) |
(4-17) |
|
|
12 – 18 years |
1000-2200 |
520-1300 |
330-920 |
110-570 |
70-480 |
|
(56-84) |
(31-52) |
(18-35) |
(6-23) |
(3-22) |
|
|
Male |
750-2240 |
450-1240 |
190-940 |
140-600 |
70-680 |
|
(52-77) |
(27-52) |
(11-40) |
(8-24) |
(4-27) |
|
|
Female |
750-2750 |
620-1990 |
210-820 |
140-450 |
60-570 |
|
(64-82) |
(33-56) |
(15-36) |
(9-20) |
(3-24) |
|
|
Male + female |
670-2180 |
480-1670 |
120-830 |
30-450 |
80-730 |
|
(54-88) |
(36-66) |
(9-41) |
(2-19) |
(6-32) |
|
