Assay

CD3, CD4 & CD8 HIV Monitoring

Key Words

CD348C, CD3, CD4, CD8, CD348, HIV

Specimen Collection

3.4ml EDTA (Red lid). For HIV monitoring only, not for diagnosis. Samples must not be refrigerated and must be <24 hours old due to white cell degradation.

Samples arriving on Friday or on a weekday that preceeds a Bank Holiday must arrive in the laboratory before 12pm or it will NOT be processed

Turnaround time

3 working days

Test indications

Monitoring CD4+ and CD8+ T lymphocyte count in patients with known HIV infection, to determine efficacy of antiretroviral medication.

Interferences

Haemolysis, clotted samples

Reference Range

See table below. Adult reference ranges from: Jentsch-Ullrich, K., et al. (2005). Lymphocyte subsets' reference ranges in an age- and gender-balanced population of 100 healthy adults—A monocentric German study. Clinical Immunology, 116(2), pp.192-197. Paediatric reference ranges from: Shearer, W., et al. (2003). Lymphocyte subsets in healthy children from birth through 18 years of age. Journal of Allergy and Clinical Immunology, 112(5), pp.973-980.

Minimum retesting interval

N/A

Additional information

Due to changes in procedure this test is currently not UKAS accredited.

Age

Cells per microlitre (% of cells)

CD3+

CD3+ CD4+

CD3+ CD8+

0 – 3 months

2500-5500

1600-4000

560-1700

(53-84)

(35-64)

(12-28)

3 – 6 months

2500-5600

1800-4000

590-1600

(51-77)

(35-56)

(12-23)

6 – 12 months

1900-5900

1400-4300

500-1700

(49-76)

(31-56)

(12-24)

1 – 2 years

2100-6200

1300-3400

620-2000

(53-75)

(32-51)

(14-30)

2 – 6 years

1400-3700

700-2200

490-1300

(56-75)

(28-47)

(16-30)

6 – 12 years

1200-2600

650-1500

370-1100

(60-76)

(31-47)

(18-35)

12 – 18 years

1000-2200

520-1300

330-920

(56-84)

(31-52)

(18-35)

Male
18 – 50 years

750-2240

450-1240

190-940

(52-77)

(27-52)

(11-40)

Female
18 – 50 years

750-2750

620-1990

210-820

(64-82)

(33-56)

(15-36)

Male + female
>50 years

670-2180

480-1670

120-830

(54-88)

(36-66)

(9-41)