Endogenous unesterified glycerol in the sample will falsely elevate serum triglycerides. Intralipid is directly measured as analyte in this assay and leads to high triglyceride results. Ascorbic acid and calcium dobesilate cause artificially low triglyceride results.

This test may require the patient to be in a fasting state. For a fasting state the patient should refrain from any foods or liquids other than water for 8hrs.

The recovery of triglyceride may be falsely low when the blood sample is taken while levels of NAC, N-acetyl-p-benzoquinone imine (NAPQI, paracetemol metabolite), and Metamizole (Novaminsulfone, Dipyrone) are present. Venipuncture should be performed prior to the administration of Metamizole. Venipuncture immediately after or during the administration of Metamizole may lead to falsely low results.